A new FRET approach demonstrates that the histone variant H2AZ stabilizes the histone octamer within the nucleosome
نویسندگان
چکیده
منابع مشابه
A new fluorescence resonance energy transfer approach demonstrates that the histone variant H2AZ stabilizes the histone octamer within the nucleosome.
Nucleosomes are highly dynamic macromolecular complexes that are assembled and disassembled in a modular fashion. One important way in which this dynamic process can be modulated is by the replacement of major histones with their variants, thereby affecting nucleosome structure and function. Here we use fluorescence resonance energy transfer between fluorophores attached to various defined loca...
متن کاملA New Fluorescence Resonance Energy Transfer Approach Demonstrates That the Histone Variant H2AZ Stabilizes the Histone Octamer within the Nucleosome*□S
Nucleosomes are highly dynamic macromolecular complexes that are assembled and disassembled in a modular fashion. One important way in which this dynamic process can be modulated is by the replacement of major histones with their variants, thereby affecting nucleosome structure and function. Here we use fluorescence resonance energy transfer between fluorophores attached to various defined loca...
متن کاملHistone H2AZ dimerizes with a novel variant H2B and is enriched at repetitive DNA in Trypanosoma brucei.
H2AZ is a widely conserved histone variant that is implicated in protecting euchromatin from the spread of heterochromatin. H2AZ is incorporated into nucleosomes as a heterodimer with H2B, by the SWR1 ATP-dependent chromatin-remodeling complex. We have identified a homolog of H2AZ in the protozoan parasite Trypanosoma brucei, along with a novel variant of histone H2B (H2BV) that shares approxim...
متن کاملP-217: Expression Analysis of The Histone Variant H2A.Z in Endometrium Tissue during The Menstrual Cycle
Background: The human endometrium undergoes cyclical changes including proliferation, differentiation, tissue breakdown and shedding (menstruation) throughout a woman’s reproductive life. The postovulatory rise in ovarian progestrone induces profound remodeling in chromatin structure of cells, and consequently differentiation of estradiol-primed endometrium. This change is crucial for embryo im...
متن کاملATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
The conserved histone variant H2AZ has an important role in the regulation of gene expression and the establishment of a buffer to the spread of silent heterochromatin. How histone variants such as H2AZ are incorporated into nucleosomes has been obscure. We have found that Swr1, a Swi2/Snf2-related adenosine triphosphatase, is the catalytic core of a multisubunit, histone-variant exchanger that...
متن کامل